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The reaction system components and procedures of Q-PCR
| components | SYBR® Premix Ex Taq™ II | forward and reverse primer (10 μM) | template DNA | sterile deionized water | total |
|---|---|---|---|---|---|
| volume | 12.5 μl | 2 μl | 1 μl | 9.5 μl | 25 μl |
| reaction procedures | predenaturation | 40 cycles | extension | ||
| denaturation | annealing | extension | |||
| Temperature (°C) | 95 | 94 | optimal temperature | 72 | 72 |
| Time (s) | 60 | 15 | 30 | 30 | 10 |
