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. 2018 Sep 10;20:207. doi: 10.1186/s13075-018-1701-1

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 8 — Representative sections of immunohistochemistry of Col II. Positive immunostaining was noted as brown staining. Black arrows indicate loss of Col II, especially in the ossification area of the cartilaginous endplate (EP) and the outer layer of the annulus fibrosus (AF) in ovariectomized (OVX) mice. n = 9 per group; scale bars = 20 μm as indicated. NP nucleus pulposus