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. 2018 Sep 10;20:207. doi: 10.1186/s13075-018-1701-1

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 9 — Immunohistochemical staining of osteopontin (OPN). DAPI stains nuclei blue and OPN expression was detected as red. Both control and ovariectomized (OVX) mice have OPN expression in the vertebral body (VB) and outer annulus fibrosus (AF), but it was almost undetectable in the nucleus. However, remarkable expression of OPN was found in the bony endplate (BEP) and outer AF in OVX mice. Meanwhile, the expression of OPN was also detected in the inner AF. n = 9 per group; scale bar = 20 μm as indicated. CEP cartilaginous endplate, EP endplate, GP growth plate, NP nucleus pulposus