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. 2018 Sep 3;24:6129–6136. doi: 10.12659/MSM.910214

Figure 3.

Figure 3

Knockdown of Wrap53 in colorectal cancer HCT116 cells promotes apoptosis and arrest at the G1 stage but inhibits tumor cell proliferation and invasion. (A) Cell proliferation assay. HCT116 cells were transfected with Wrap53 shRNA or negative control shRNA, and proliferation was assessed by MTT assay (*** P<0.001; t test at day 5). (B) Cell apoptosis assay. HCT116 cells were transfected with Wrap53 shRNA or negative control shRNA and Annexin V staining was assessed by flow cytometry (*** P<0.001; t test). (C) Cell cycle assay. HCT116 cells were transfected with Wrap53 shRNA or negative control shRNA and PI staining was assessed by flow cytometry (* P<0.05, ** P<0.01, *** P<0.001; t test). (D) Transwell invasion assay. HCT116 cells were transfected with Wrap53 shRNA or negative control shRNA and invasion was assessed by Transwell invasion assay (*** P<0.001; t test). All experiments were performed in triplicate.