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. 2018 Sep 4;12:288. doi: 10.3389/fncel.2018.00288

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Copyright © 2018 Shen, Gu, Liu, Xu, Qian, Zhang, Zhou, Guan, Sun, Wang and Jin.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effect of 2-h ischemia on MMP-2/9 induction. MMP-2/9 was induced in ischemic brain tissue after 2-h ischemia. (A) Representative gelatin zymogram showed MMP-2/9 levels in the non-ischemic (NI) and ischemic (I) hemispheric tissue. MMP-2 bands were much stronger than MMP-9 bands on zymogram gels. STD is a mixture of standard MMP-2/9. The relative band intensity of MMP-2 (B) and MMP-9 (C) was quantified. A significant increase was observed for MMP-2 in the ischemic tissue after 2-h middle cerebral artery occlusion (MCAO; *P < 0.05 vs. NI, n = 5), while there was no significant increase for MMP-9 (P > 0.05 vs. NI, n = 5). Data were expressed as mean ± SEM.