REAGENT or RESOURCE | SOURCE | IDENTIFIER |
---|---|---|
Antibodies | ||
A full list of antibodies is provided in Table S1 | ||
Biological Samples | ||
TNBC TMA was compiled by the Stanford Pathology Department |
||
Chemicals, Peptides, and Recombinant Proteins | ||
TBS IHC Wash Buffer with Tween 20 | Cell Marque | Cat#935B-09 |
PBS IHC Wash Buffer with Tween 20 | Cell Marque | Cat#934B-09 |
Target Retrieval Solution, pH 9, (3:1) | Agilent (Dako) | Cat#S2375 |
Avidin/Biotin Blocking Kit | Biolegend | Cat#927301 |
Gelatin (cold water fish skin) | Sigma-Aldrich | Cat#G7765–250 |
Xylene Histological grade | Sigma-Aldrich | Cat#534056–500 |
Glutaraldehyde 8% Aqueous Solution EM Grade | EMS | Cat#16020 |
Normal Donkey serum | Sigma-Aldrich | Cat#D9663–10ML |
Bovine Albumin (BSA) | Fisher | Cat#BP1600–100 |
Centrifugal filters (0.1 μm) | Millipore | Cat#UFC30VV00 |
Critical Commercial Assays | ||
Maxpar X8 Antibody labeling kit | Fluidigm | Cat#2011XXX |
MIBItag Conjugation Kit | IONpath | Cat#600XXX |
ImmPRESS UNIVERSAL (Anti-Mouse/Anti-Rabbit) IgG KIT (HRP) |
Vector Laboratories | Cat#MP-7500–15 |
ImmPACT DAB (For HRP Substrate) | Vector Laboratories | Cat#SK-4105 |
Deposited Data | ||
https://mibi-share.ionpath.com | All the data of the study |
|
Software and Algorithms | ||
Data analysis was done using Matlab 2016 | Mathworks | |
https://github.com/lkeren/MIBIAnalysis | Analysis code | |
https://hub.docker.com/r/vanvalen/deepcell-mibi | Segmentation code |