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. Author manuscript; available in PMC: 2019 Sep 6.
Published in final edited form as: Cell. 2018 Sep 6;174(6):1373–1387.e19. doi: 10.1016/j.cell.2018.08.039

Key resources table

 REAGENT or RESOURCE  SOURCE  IDENTIFIER
 Antibodies
 A full list of antibodies is provided in Table S1
 Biological Samples
 TNBC TMA was compiled by the Stanford Pathology
 Department
 Chemicals, Peptides, and Recombinant Proteins
 TBS IHC Wash Buffer with Tween 20  Cell Marque  Cat#935B-09
 PBS IHC Wash Buffer with Tween 20  Cell Marque  Cat#934B-09
 Target Retrieval Solution, pH 9, (3:1)  Agilent (Dako)  Cat#S2375
 Avidin/Biotin Blocking Kit  Biolegend  Cat#927301
 Gelatin (cold water fish skin)  Sigma-Aldrich  Cat#G7765–250
 Xylene Histological grade  Sigma-Aldrich  Cat#534056–500
 Glutaraldehyde 8% Aqueous Solution EM Grade  EMS  Cat#16020
 Normal Donkey serum  Sigma-Aldrich  Cat#D9663–10ML
 Bovine Albumin (BSA)  Fisher  Cat#BP1600–100
 Centrifugal filters (0.1 μm)  Millipore  Cat#UFC30VV00
 Critical Commercial Assays
 Maxpar X8 Antibody labeling kit  Fluidigm  Cat#2011XXX
 MIBItag Conjugation Kit  IONpath  Cat#600XXX
 ImmPRESS UNIVERSAL (Anti-Mouse/Anti-Rabbit) IgG
 KIT (HRP)
 Vector Laboratories  Cat#MP-7500–15
 ImmPACT DAB (For HRP Substrate)  Vector Laboratories  Cat#SK-4105
 Deposited Data
https://mibi-share.ionpath.com  All the data of the
 study
 Software and Algorithms
 Data analysis was done using Matlab 2016  Mathworks
https://github.com/lkeren/MIBIAnalysis  Analysis code
https://hub.docker.com/r/vanvalen/deepcell-mibi  Segmentation code