. 2018 Sep 4;9:2094. doi: 10.3389/fmicb.2018.02094

Table 1.

Fermentation parameters at day 14 (experimental period) and day 18 (/17 withdrawal period).

Parameter	day	Treatment				Pooled SD	P-value
		CON	LD	HD	MON		Treatment	Time	Interaction
pH	14	6.79^AB	6.66^A	6.66^A	6.87^B	0.05	0.0016²	<0.001	0.072
	18	6.85^AB	6.82^A	6.79^A	6.90^B	0.04
Redox potential	14	−264^Ab	−267^Ab	−300^A	−244^B∗	19.9	n.s.	0.036	0.0088
	18	−291	−280	−271	−288^∗	12.8
NH₃-N [mM]	14	6.30	6.74	9.94	6.02	1.01	n.s.	n.s.	0.078
	18	7.80	7.41	6.54	6.69	1.56
SCFA total [mmol/d]	14	21.21^AC	30.86^AB	36.31^B	16.46^C	4.10	<0.001	0.0042	n.s.
	18	18.24^AC	20.71^AB	24.50^B	15.38^C	3.39
Acetate [%]	14	59.00^A	56.66^A	54.59^A	51.49^B	1.67	<0.001	0.0096	0.076
	18	58.34^A	58.91^A	58.46^A	52.97^B	1.01
Propionate [%]	14	25.28^A	24.90^AB	22.54^B	38.43^C	1.24	<0.001	n.s.	n.s.
	18	25.64^A	24.78^AB	23.58^B	37.20^C	0.80
Butyrate [%]	14	10.57^A	12.24^Ab∗	15.07^B∗	4.04^C	1.96	<0.001	0.0010	<0.001
	18	10.85^α	10.78^α∗	11.52^α∗	4.32^β	1.27
Isovalerate [%]	14	2.37^A	2.68^A	4.25^B∗	n.d.	0.53	0.029	0.0010	0.0082
	18	2.24	2.25	2.87^∗	n.d.	0.38
Valerate [%]	14	2.78^A	3.51^A	3.57^A	6.04^B	0.55	<0.001	n.s.	n.s.
	18	2.94^A	3.28^A	3.57^A	5.51^B	0.54
Net H₂ production [mM]¹	14	36.55^A	51.07^AB	60.89^B	16.43^C	4.75	<0.001	0.031	0.074
	18	30.16^A	34.41^AB	42.41^B	17.07^C	6.64
Degradation of organic matter [%]	14	41.96^Ab	40.90^Ab	50.44^A	31.09^B∗	5.63	0.0046	n.s.	0.022
	17	42.30	40.47	37.76	35.95^∗	2.84

¹Net H₂ production was estimated by stoichiometric calculation using the formula by (Demeyer, 1991; Wang et al., 2014). ²Results of the statistical analysis of pH, redox potential and NH₃-N have been included in a conference abstract previously (Eger et al., 2018). During the experimental period control fermenters (CON) received no addition, low dose group (LD) was treated with 1 g of the experimental mixture, high dose fermenters (HD) received 2 g of the experimental mixture and monensin was used as positive control (MON). During withdrawal period no additions were made. Significant differences (at least P < 0.05) are indicated by different capital letters among treatment groups for both time-points, by small letter among treatment groups at day 14 and by Greek letters among treatment groups at day 18. Asterisks indicate significant differences within treatment group between time-points. Data are presented as means and pooled SD. n.d. = not detectable.