Figure 2.
Mitochondrial function is impaired during unloading and improves following 3 days of recovery only in adult mice. (A) Complex I (Glutamate and Malate) supported LEAK (CIL or State 4) respiration. (B) Palmitoylcarnitine supported OXPHOS (State 3) respiration. (C) Complex I supported OXPHOS respiration. (D) Complex I & II supported OXPHOS respiration. (E) Mitochondrial maximal H2O2 emission as determined with Amplex Red, during oligomycin-induced state 4 respiration. (F) Mitochondrial calcium retention capacity. n = 8–10 per group for permeabilized fiber bundle experiments. (G) Total OXPHOS protein content by immunoblot, n = 5 per group. (H) Total cardiolipin content by tandem mass spectrometry. (I) Heatmap depicting change in individual immature, mature, and remodeled cardiolipin species. The normalized (z-score) mean of each row (cardiolipin species) was visualized in the heatmap, n = 7 per group. (J) Representative immunoblot for OXPHOS proteins. Data are presented as mean ± SEM. **p <0.05 ANOVA/Bonferroni correction. *p <0.05 versus control ANOVA/Bonferroni correction. ANOVA = analysis of variance.