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. 2018 Sep 6;11(6):1343–1349. doi: 10.1016/j.tranon.2018.08.009

Figure 3.

Figure 3

Image representative of SKBR3 cells treated with APR-246 and stained with PAb1620 (A) to detect wild-type-p53 or PAb240 (B) to detect mutant p53. DAPI nuclear stain was used as a control. All experiments were carried out in triplicate. (C) Histogram representatives of SKBR3 cells treated with APR-246 and stained with PAb1620 to detect WT-p53. (D) Bar chart representation of Pab1620 staining measured by flow cytometry and analyzed using FlowJo v.10 software. Data were analyzed using paired t test. (E) Bar chart representation of absolute p53 protein levels quantified using the PathScan p53 ELISA kit.