Skip to main content
. Author manuscript; available in PMC: 2018 Sep 11.
Published in final edited form as: Mod Pathol. 2017 Dec 1;31(2):214–234. doi: 10.1038/modpathol.2017.156

Figure 4.

Figure 4

The Immunoscore is a standardized approach to characterizing T-cell infiltration of surgical pathology tumor specimen. Left panels: The border between the advancing tumor edge and normal tissue is annotated on a colorectal carcinoma specimen. A 500-μm distance on either side of this border is designated the ‘invasive margin’ (IM, yellow region). The remainder of the tumor is designated as the ‘tumor core’ (TC, blue region). Upper right panels: Immunohistochemistry for CD3 and CD8 is used to quantify cell densities for each of these immune cell subsets in both the invasive margin and tumor core. Lower right panels: The density of each region is labeled ‘high’ or ‘low’ density for each marker. The mean percentile of the four immune parameters is calculated, resulting in a possible Immunoscore ranging from I0 to I4, or three categories (Low, Intermediate, High). (Abbreviations: IM, invasive margin; TC, tumor core).