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. 2018 Aug 6;28(10):3724–3739. doi: 10.1093/cercor/bhy180

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© The Author(s) 2018. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 4. — Comparison of RRBS and bisulfite pyrosequencing results for PROSER2 (interspecific neuronal DMR), CAMTA1 (interspecific non-neuronal DMR), RBFOX3 (intraspecific human DMR), and RTN4R (intraspecific chimpanzee DMR). Thin blue bars in the RRBS plots indicate the methylation levels (0–100%) of single CpGs. The X-axis indicates the genomic position of the identified DMR, which is highlighted by a pink background. The gray line represents the sequence coverage (number of reads for each nucleotide). The bar diagrams below the RRBS plots indicate the single CpG methylation levels of a given region measured by bisulfite pyrosequencing. Significance levels for an inter- or intraspecific methylation difference are indicated by one star (P < 0.05) and two stars (P < 0.01), respectively. Identical CpGs in the RRBS and bisulfite pyrosequencing data are framed in red.