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. 2018 Aug 28;9(67):32736–32750. doi: 10.18632/oncotarget.25127

Figure 5. Kras-PKCι-YAP1 signaling pathway exists in Kras mutant pancreatic cancer cells and is crucial for the survival of these cells.

Figure 5

(A) Immunoblot and (B) qRT-PCR analyses of Kras, PKCι, YAP1 and Mcl-1 expression in HPDE6-C7 expressing control shRNA or shRNA-kras (Error bar, ± SD. n = 3. *p < 0.05). (C) Immunoblot and (D) qRT-PCR analyses of Kras, PKCι, YAP1 and Mcl-1 expression in MiaPaCa and PANC-1 with Kras knockdown, or Kras knockdown in presence of PKCι overexpression. MiaPaCa and PANC-1 transfected with control plasmids (plasmid expressing control shRNA and void pCMV-HA) are used as controls (Error bar, ± SD. n = 3. *p < 0.05 compared with control samples. **p < 0.05 compared with Kras knockdown samples without PKCι overexpression). (E) Upper panels: Immunoblot detection of YAP1 overexpression in MiaPaCa and PANC-1 cells transfected with pCMV-YAP1 or void vector pCMV-HA (Control). Lower panel: Quantification of apoptotic cells in MiaPaCa and PANC-1 treated with control diluent, or with 0.1 mM ATM in or not in presence of YAP1 overexpression (Error bar, ± SD. n = 5. *p < 0.05 compared with control cells. **p < 0.05 compared with ATM-treated cells without YAP1 overexpression). (F) Upper panels: Immunoblot analysis of YAP1 expression in MiaPaCa and PANC-1 cells expressing lentiviral shRNA-Ctrl or shRNA-yap1. Lower panel: Quantification of apoptotic cells in MiaPaCa and PANC-1 infected with lentivirus producing shRNA-Ctrl or shRNA-yap1 (Error bar, ± SD. n = 5. *p < 0.05).