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Anisomycin upregulates utrophin protein in vitro. (a) C2C12 myoblasts treated with 1 nM anisomycin or vehicle (DMSO) and harvested at 24 hours (n = 9). (b) Mdx primary myoblasts treated with 1 nM anisomycin or vehicle (DMSO) and harvested at 24 hours (n = 4). Immunoblotting for utrophin shows dramatic upregulation with respect to the housekeeper protein tubulin. Error bars show SEM of average (*P < 0.01, two‐tailed unpaired Student's t‐test).
