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. 2018 Jun 19;11(5):513–522. doi: 10.1111/cts.12569

Figure 5.

Figure 5

In vitro glucuronidation of testosterone, A and Etio using human liver microsomes (HLMs) and recombinant human UGT2B enzymes. (a‐c) These panels represent the correlation between UGT2B17 protein expression in HLM and glucuronidation activity toward testosterone, A and Etio, respectively. (d‐f) These panels represent the correlation between UGT2B7 protein expression in HLM and glucuronidation activity toward testosterone, A and Etio, respectively. (g) This panel represents glucuronidation activity of recombinant human UGT2B enzymes toward testosterone, A and Etio at 1 μM. (h and i) These panels show the estimated glucuronidation activity of UGT2Bs toward testosterone, A, and Etio in the human liver and the intestinal microsomes. The scaling was based on UGT2B protein abundance in the liver (36.6, 21.2, and 0.92 pmol/mg36 and the intestine (2.83, 0.0, and 8.87 pmol/mg) for UGT2B7, UGT2B15, and UGT2B17, respectively.34 AG, androsterone glucuronide; EtioG, etiocholanolone glucuronide; TG, testosterone glucuronide