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. 2018 Jul 14;102(18):7725–7738. doi: 10.1007/s00253-018-9200-5

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© The Author(s) 2018

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Fig. 2 — Sequence alignment of IspSs from several organisms. The blue and red rectangle indicates the two identified active site lid motifs and the two conserved metal-binding motifs. Seven residues that have been identified as having special enzyme activities are indicated with small black triangles. The amino acid mutations applied in research studies are also indicated. The accession numbers of the IspSs listed are CAC35696.1, AB198180.1, ADV58934.1, MH084470, MH084471, ACD70404.1, and BAF02831.1