Fig. 8.

Cell migration assay performed with SH-SY5Y cells. a Cell migration assay using the SH-SY5Y neuroblastoma cell line. SH-SY5Y cells either transfected with Nox4 or scrambled small iRNA (siRNA) were grown to 80% confluency in a 24-well plate containing a hydrogel spot (time point 0 h). After removing the spot, the cells transfected with scrambled siRNA but not the Nox4 siRNA started to migrate into the cell free area (time point 24 h). b The remaining free area in the open spots was quantified. This shows that mobility of the neuroblastoma cells depends on Nox4 activtiy. c Under similar conditions, the actin cytoskeleton regulator neuronal Wiskott-Aldrich syndrome protein (N-WASP) is down-regulated as shown by Western blots normalized to the housekeeping protein glyceraldehyde-3-phosphate dehydrogenase (GAPDH) in the cancer cell line HepG2. C control (scrambled siRNA), N4 Nox4 specific siRNA