Fig. 7.

Increased dendritic complexity, increased length and reduced spine density of DG granule cells in Bsn cKO mice. Example images of Golgi impregnation of dentate gyrus (DG) granule cells from WT (a) and Bsn cKO mice (a′) [high magnification of entangled area in lower panel showing spine density from WT (b) and cKO mice (b′)]. c Sholl analysis of apical dendrites shows an increased number of intersections in cKO mice compared to WT mice, indicating an enhanced dendritic arborization in cKO in a region 60–120 µm away from soma and d increased cumulative length of dendrites in cKO mice compared to WT mice (WT: N = 5 mice, n = 10 cells; cKO: N = 6 mice, n = 11 cells). e The density of spines measured in the region of increased dendritic arborization in cKO is reduced when compared WT littermates (N = 4 mice each; WT: n = 7 cells, cKO: n = 8 cells). Analysis of pyramidal cells in the CA1 region of WT (f) and cKO (f′) mice reveals no change in g, arborization of basal and apical dendrites or h, total dendritic branch length. Scale bar in a, f is 15 µm and in b is 5 µm. All values are mean ± SEM; *p ≤ 0.05, **p ≤ 0.01, two-way repeated measures ANOVA (c, g, h) and Student’s t test (d, e)