Fig. 1.

Schematic representation of the experimental outline. The horizontal black arrow indicates the time line starting with the beginning of the cuprizone diet, which lasted for 35 days (5 weeks; red arrow). At this point, the animals were re-introduced to normal food till the end of the experiments. The different experimental groups were tested in vitro and in vivo, at the end of the cuprizone diet (full demyelination—blue inset) and at the early (yellow inset) and late phases (orange inset) of remyelination, namely 7 and 25 days after re-introduction of normal food. In addition, the horizontal color-coded lines indicated the experimental outline for the treatment with DMF. Treatment started when normal food was re-introduced to the diet and it lasted for 7 or 25 days. The group of DMF-treated animals was compared to vehicle-treated or non-injected mice, which underwent the same handling