Fig. 7.

DMF administration ameliorates the auditory cortical functioning in vivo. a Schematic representation of the Pavlovian fear conditioning paradigm: animals were presented with the two tones of 2.5-and 10-kHz frequency, respectively. The 10-kHz tone was associated to a mild foot-shock (conditioned stimulus—indicated by the yellow flash sign in the third box from the left). The animals were adapted to the two tones on day 1 by random presentation of the tone twice per day. During the second day, the 10-kHz frequency was associated with a foot-shock. The third day, animals were randomly presented with the two tones, without any foot-shock and the reaction to the conditioned stimulus was quantified as immobility behavior (freezing—indicated by the wave sign). b Bar graphs showing the percentage of freezing in response to 2.5 and 10 kHz in the control group (black bars) and in cuprizone-treated animals (blue bars). c Bar graphs showing the percentage of freezing in response to 2.5 and 10 kHz in animals treated with DMF for 7 days after remyelination starts (dark orange bars) in comparison to vehicle-treated and non-injected animals (dark red and yellow bars, respectively). d Bar graphs showing the percentage of freezing in response to 2.5 and 10 kHz in animals treated with DMF for 25 days after remyelination starts (olive green bars) in comparison to vehicle-treated and non-injected animals (light brown and pink bars, respectively). ***p < 0.001, 2.5 vs. 10 kHz