Fig. 9.

Effect of compounds 3i-1000 and 3i-1047 on caspase activation in hiPSCs. After a 4-h compound exposure, the cells were incubated with a detection reagent for activated Caspase-3/7 for 50 min and the nuclei were stained with Hoechst for 10 min. Fixed or live cells were imaged with CellInsight High Content Analysis Platform. a Representative images from four independent experiments are shown. Adjustments of individual color channels to enhance brightness and contrast were made identically to all representative images. b Quantification of the proportion of cells positive for fluorescent caspase 3/7 activity reporter is expressed as mean + SEM (n = 6). **P < 0.01 vs. DMSO (Welch ANOVA followed by Games–Howell)