Fig. 4.

Effect of α-tocopherol treatment on blood–brain barrier in control and kainate-induced epileptic rats. Western blot analysis of the expression levels of claudin-5, used as marker of blood–brain barrier integrity, in hippocampal homogenates obtained from rats of the experimental groups: αK, treated kainate-exposed; VK, untreated kainate-exposed; αC, treated non-epileptic; VC, untreated non-epileptic. Per each experimental group: representative immunoblots are displayed and anti-actin blots are shown as loading control. Note that immunoblots are shown in the same sequence as bars in the corresponding histograms. Histograms represent densitometric analyses of blots from three independent experiments (means ± SEM). Statistical analyses performed by one-way ANOVA and Tukey’s post hoc test: **p < 0.01