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. 2018 Feb 27;55(10):7921–7940. doi: 10.1007/s12035-018-0911-5

Fig. 5.

Fig. 5

Female Ambra1+/− mice show reduced inhibitory synaptic transmission onto CA1 pyramidal neurons. a Schematic of a dorsal hippocampus slice showing the positioning of the stimulating and recording electrodes during recordings of dendritic (left) and perisomatic eIPSCs (right). b Examples of dendritic eIPSCs at half-maximal Schaffer collateral stimulation intensity from a female WT and Ambra1+/− CA1 pyramidal neuron (− 70 mV; scale bars: 100 ms, 100 pA), and mean input/output curve (± sem) for eIPSCs (n = 17 WT and 16 Ambra1+/− cells) in response to increasing afferent stimulation. eIPSCs are reduced in Ambra1+/− females (two-tailed unpaired t test for the indicated intensities: *P < 0.050, **P < 0.010). c Traces of perisomatic eIPSCs from CA1 pyramidal neurons (− 70 mV; scale bars: 20 ms, 100 pA) recorded at half-maximal stimulation in the stratum pyramidale in the presence of WIN55,212-2 (1 μM), and mean input/output curve (± sem; n = 8 WT and 7 Ambra1+/− cells) showing that perisomatic eIPSCs are reduced in Ambra1+/− females (two-tailed unpaired t test for the indicated intensities: *P < 0.050, **P < 0.010). d Example paired-pulse responses (100 ms interval) of perisomatic eIPSCs from a female WT and Ambra1+/− pyramidal neuron (− 70 mV; scale bars: 50 ms, 100 pA) at half-maximal stimulation in the stratum pyramidale in the presence of WIN55,212-2. The PPR (± sem) is increased in Ambra1+/− females (two-tailed unpaired t test for the indicated intervals: *P < 0.050, **P < 0.010; n = 8 WT and 7 Ambra1+/− cells). e Representative mIPSCs from female WT and Ambra1+/− pyramidal neurons (− 70 mV; scale bars: 100 ms, 20 pA). f, g The mean mIPSC amplitude is unchanged in female Ambra1+/− mice (f), but the IEI (g) is reduced (n = 12 WT and 13 Ambra1+/− cells; two-tailed unpaired t test: **P = 0.003)