. 2018 May 29;75(10):1256–1266. doi: 10.1007/s00284-018-1518-9

Table 2.

Multiplex PCR^*. Primer sequence of staphylococcal phage type, PCR product length and type of protein

Phage type	Primer***	Primer sequence (5′–3′)**	PCR product length (bp)	Sequence coding for
3A-like phage	SGA1	TATCAGGCGAGAATTAAGGG	744	Tail fibres
3A-like phage	SGA2	CTTTGACATGACATCCGCTTGAC	744	Tail fibres
11-like phage	SGB1	ACTTATCCAGGTGGYGTTATTG	405	Hypothetical tail protein
11-like phage	SGB2	TGTATTTAATTTCGCCGTTAGTG	405	Hypothetical tail protein
77-like phage	SGF1	CGATGGACGGCTACACAGA	155	Hypothetical tail protein
	SGF2	TTGTTCAGAAACTTCCCAACCTG	155	Hypothetical tail protein
	SGFa1	TACGGGAAAATATTCGGAAG	548	Packaging protein
	SGFa2	ATAATCCGCACCTCATTCCT	548	Packaging protein
	SGFb1	AGACACATTAAGTCGCACGATAG	147	Packaging protein
	SGFb2	TCTTCTCTGGCACGGTCTCTT	147	Packaging protein
187-like phage	SGL1	GCTTAAAACAGTAACGGTGACAGTG	648	Hypothetical capsid protein
187-like phage	SGL2	TGCTACATCATCAAGAACACCTGG	648	Hypothetical capsid protein
Twort-like phage	SGD1	TGGGCTTCATTCTACGGTGA	331	Major capsid protein
Twort-like phage	SGD2	GTAATTTAATGAATCCACGAGAT	331	Major capsid protein

*S. aureus strain NCTC 8325 was used as positive control

**Nucleotide sequences were derived from the published sequences by Pantůček et al [31]

***The concentration of primers was 0.04 µmol