Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Jul 13;67(9):1425–1436. doi: 10.1007/s00262-018-2204-2

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

PMC Copyright notice

Fig. 2 — Stimulation of co-cultured autologous mDCs and pDCs enhance pR-mediated mDC, but not pDC, maturation. Purified autologous mDCs and pDCs were cultured overnight either alone or together in a 5:1 or 1:1 ratio in the presence of medium alone/IL-3 or pR complexes. The total number of cells was kept constant between the cultures. Upregulation of maturation marker CD80 was evaluated by flow cytometry. Cytokine release was analyzed with ELISA. a mDCs or b pDCs from 11 donors cultured either alone or combined at a 5:1 or 1:1 ratio in the presence or absence of stimuli. Average secreted levels of c TNF-α ± SEM from eight donors, d IL-12p70 ± SEM from seven donors, and e IFN-α ± SEM from ten donors. Statistical differences within the treatment groups were analyzed by paired one-way ANOVA with Bonferroni’s multiple comparison test and significance is indicated by **(p < 0.01), ***(p < 0.001), or ns (non-significant)