Fig. 4.

Limited variance of ISC-marker gene expression profile among cells constituting patient-derived small intestinal organoids. a Comprehensive principal component analysis (PCA) of the total single-cell gene expression data acquired from small intestinal organoids. Data shows distributions of gene expression variance at the single-cell level, after elimination of outlier cells (n = 1037). Color key indicates each patient-derived small intestinal organoid. b The same analysis shown in (a) displaying single-cell level gene expression variance of each small intestinal organoid group. c Violin plot showing single-cell gene expression level (Y-axis), and cell frequency (X-axis) in small intestinal organoids