Table 2.

Primers used in the multiplex PCRs for amplification of edible insect species

Insect species	Target gene	Primer sequence (5′-3′)	Annealing Temperature (°C)	Amplicon size (bp)
Multiplex PCRI
Locusta migratoria migratorioides	16S rRNA	CAAAGGTAGCATAATCATTAGT CTCCGGTTTGAACTCAGATC	55°C	370–420
Tenebrio molitor	16S rRNA	CAAAGGTAGCATAATCATTAGT AGTTAAATAAATTTTCTAACCG	55°C	240
Multiplex PCRII
Acheta domesticus	16S rRNA	CAAAGGTAGCATAATCATTAGT TCATTCCATAATACAGGATCA	55°C	290
Bombyx mori	16S rRNA	CAAAGGTAGCATAATCATTAGT CGTCTTTTTAAATAATTTTAACT	55°C	134
Multiplex PCRIII
Alphitobius diaperinus	COI	AGTAGGAATAGACGTAGATACTCG TAGCAAATACGGCTCCTATTGAT	55°C	284
Schistocerca gregaria	16S rRNA	CAAAGGTAGCATAATCATTAGT TCATTAAATATAGAAAGACAAACA	55°C	198
Zophobas atratus	COI	GGGCATCAGTCGATCTCGCA CGATCAAAAGTTATTCCTTGTGG	55°C	120
Multiplex PCRIV
Gryllodes sigillatus	16S rRNA	CAAAGGTAGCATAATCATTAGT TATTTAAGTCATAATACTATCCT	50°C	195
Galleria mellonella	COI	TATTAATATAAAATTAAATGGTT GAACAGGCAATGAAAGAAG	50°C	100