Figure 3.

EGF induces protein stabilization of Snail.

(A) Western blot analyzes for Snail in SACC-83 cells treated with 50 ng/mL EGF for different time intervals for 4 hours.(B) Western blot analyzes for Snail from SACC-83 cells treated with 0 to 200 ng/mL EGF for 2 hours.(C) RT-PCR analyzes for mRNA levels of Snail for different time intervals.(D) RT-PCR analzses for mRNA levels of Snail from SACC-83 cells treated with 0 to 200 ng/mL EGF for 2 hours.(E-F) SACC-83 cells were treated with or without EGF for 2 hr, followed by incubation with cycloheximide (CHX; 10 μM) for an extended period of time. The levels of Snail were determined by western blot analysis.(G) Graphic representation of densitometry results for Snail after cycloheximide treatment (circle, with EGF; square, without EGF).(H) Immunofluorescence staining for Snail in SACC-83 cells treated with or without 50 ng/mL EGF for 2 hours.