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EGF inhibits the ubiquitination of Snail.
(A) HA-ubiquitin was expressed in Snail/HEK293 cells followed by treatment with or without EGFand MG-132 for 2 hr. Ubiquitin was immunoprecipitated from equal amount of lysates and the ubiquitinated Snail was examined by western blotting.(B) SACC-83 cells were either treated with or without EGF. Endogenous Snail was immunoprecipitated, and the bound of ubiquitin was detected by western blotting.
