Figure 3.

Effect of ChREBP gene knockout on body, liver, and metabolic parameters in mice subjected to AKT overexpression by hydrodynamic gene transfer. Control (injected with empty vector; n = 10) and AKT/ChREBP mice (n = 15) were sacrificed 29 weeks post injection (w.p.i.). AKT/ChREBPKO mice (n = 15 for each group) were sacrificed at two distinct time points: 29 w.p.i. (indicated as AKT/ChREBPKO I) and 52 w.p.i. (AKT/ChREBPKO II), respectively. Tukey–Kramer test: P < 0.001 a, vs control; b, vs AKT/ChREBP; c, vs AKT/ChREBPKO I mice. Abbreviations: FA, fatty acids; ENO, enolase activity; G6PDH, glucose 6-phosphate dehydrogenase activity; LDH, lactate dehydrogenase activity; TG, triglycerides.