Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Jul 18;17(12):1496–1512. doi: 10.1080/15384101.2018.1489182

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2018 Informa UK Limited, trading as Taylor & Francis Group

PMC Copyright notice

Figure 8. — Chrebp silencing by specific siRNA for 48h induces downregulation of Fasn and Scd1 targets and cooperates with the MEK inhibitor U0126 to downregulated Plk1, Skp2, and Foxm1 genes (as determined by quantitative real-time RT-PCR) in the AKT/Ras mouse HCC cell line. Quantitative values for Chrebp, Fasn, Scd1, Plk1, Skp2, and Foxm1 mRNA levels were calculated by using the PE Biosystems Analysis software and expressed as Number target (NT). NT = 2^−ΔCt, wherein ΔCt value of each sample was calculated by subtracting the average Ct value of the target gene from the average Ct value of the β-Actin gene (housekeeping gene). Experiments were conducted three times in triplicate. Tukey–Kramer test: at least P < 0.001 a, vs scrambled siRNA; b, vs DMSO (solvent); c, vs U0126; d, vs ChREBP siRNA.