Fig. 2.

Subcellular localization of m-THPC in SW480 (A–L) and SW620 (a–l) cells. Cells were incubated with 0.74 μM m-THPC for 8 h, then stained with 1 μmol/l endoplasmic reticulum marker (A–D: SW480, a–d: SW620), 50 nmol/l lysosome marker (E–H: SW480, e–h: SW620), and 100 nmol/l mitochondrion marker (I–L: SW480, i–l: SW620) at 37 °C for 30 min. Photographs were taken by confocal laser scanning microscopy. A, E, I, a, e, and i: m-THPC autofluorescence (red); B and b: endoplasmic reticulum marker (green); F and f: lysosome marker (blue); J and j: mitochondrion marker (green); C, G, K, c, g, and k: merged of A and B, E and F, I and J, a and b, e and f, and i and j, respectively; D, H, L, d, h, and l: phase contrast