Table 2.
Instrumental parameters for ICP–MS and SEC–ICP–MS measurements including chromatographic conditions
| ICP–MS Agilent 7500ce | SEC–ICP–MS Agilent 8800 | |
|---|---|---|
| RF power/W | 1560 | 1350 |
| Cones | Ni | Ni |
| Registered isotopes | 101Ru, 102Ru, 115In, 185Re, 189Os, 192Os | 48SO, 72Fe, 101Ru, 102Ru, 189Os, 192Os, 206OsO, 224OsO2, 240OsO3, 256OsO4 |
| Dwell time/s | 0.3 | – |
| Replicates | 10 | 1 |
| Carrier gas (Ar)/dm3 min−1 | 0.92–0.97 | 1.1 |
| Make up gas (Ar)/dm3 min−1 | 0.22–0.27 | – |
| Plasma gas (Ar)/dm3 min−1 | 15 | 15 |
| Reaction gas (O2)/cm3 min−1 | – | 0.3 (30%) |
| HPLC column | – | ACQUITY UPLC Protein BEH SEC, 125 Å, 1.7 µm, 4.6 mm × 150 mm |
| Eluent | – | 50 mM CH3COONH4 (pH 6.8) |
| Flow rate/cm3 min−1 | – | 0.3 |
| Injection volume/mm3 | – | 2 |
| Column temperature/°C | – | 30 |
| Autosampler temperature/°C | – | 4 |