Table 1.
The mobile phases used in column chromatography and calculated values of the retention factor
| Compound | Mobile phase | Ratio | Retention factor Rf |
|---|---|---|---|
| 5a | CH2Cl2–EtOH | 60:1 | 0.39 |
| 5b | CH2Cl2–EtOH | 40:1 | 0.55 |
| 5c | CH2Cl2–EtOH | 60:1 | 0.32 |
| 5d | CH2Cl2–EtOH | 60:1 | 0.43 |
| 5e | CHCl3–EtOH | 5:1 | 0.68 |
| 5f | CHCl3–EtOH | 5:1 | 0.18 |
| 5g | CH2Cl2–EtOH | 40:1 | 0.43 |
| 5h | CHCl3–EtOH | 15:1 | 0.45 |
| 5i | CHCl3–EtOH | 5:1 | 0.73 |
| 6a | CH2Cl2–EtOH | 40:1 | 0.43 |
| 6b | CH2Cl2–EtOH | 40:1 | 0.49 |
| 6c | CH2Cl2–EtOH | 60:1 | 0.36 |
| 6d | CH2Cl2–EtOH | 60:1 | 0.37 |
| 6e | CHCl3–EtOH | 15:1 | 0.24 |
| 6f | CHCl3–EtOH | 5:1 | 0.22 |
| 6g | CH2Cl2–EtOH | 60:1 | 0.31 |
| 6h | CHCl3–EtOH | 15:1 | 0.32 |
| 6i | CHCl3–EtOH | 5:1 | 0.74 |
| 6j | CHCl3–EtOH | 5:1 | 0.20 |