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. 2018 Aug 22;61(10):2087–2097. doi: 10.1007/s00125-018-4656-5

Fig. 5.

Fig. 5

(a) Time course of Me-4FDG excretion into the urinary bladder of a rat. Me-4FDG (300 MBq) was injected intravenously into rats and excretion into the urinary bladder measured using microPET. Its excretion into the urinary bladder (MBq) is plotted as a function of time before and after injection of 1 mg/kg dapagliflozin (Dapa; an SGLT2 inhibitor) at 20 min. Injection of dapagliflozin causes the rapid excretion of Me-4FDG into the urinary bladder. Similar results were obtained with intravenous phlorizin (A. S. Yu and C. Ghezzi, unpublished results). (b) SGLT2 distribution in a rat, analysed using F-Dapa microPET. Animals were injected with F-Dapa (300 MBq) and the distribution of the tracer was imaged at 60 min using microPET. F-Dapa binding to organs is shown in control conditions (white bars) or after competition with cold dapagliflozin (black bars). Three-dimensional regions of interest (ROIs) were drawn over each organ and the data are presented as a percentage of the initial dose per tissue weight (%ID/g), with the exception of the bladder, for which values are presented as percentage ID per total bladder volume. Data are presented as the apparent density of SGLT2 in each organ, as means + SEM. ***p ≤ 0.001 vs control. The data show that functional SGLT2 is only expressed in the kidney. (c, d) Location of SGLT2 in the mouse kidney, visualised using F-Dapa and ex vivo microautoradiography and H&E staining. A mouse was injected with 148 MBq F-Dapa and after 15 min the kidney was removed and processed. (c) Aligned autoradiogram and H&E-stained image of the whole mouse kidney. Scale bar, 1 mm. (d) F-Dapa binding to the tubules surrounding a glomerulus. These images show that dapagliflozin is filtered by glomeruli in the outer renal cortex and then binds to SGLT2 in the early proximal tubule. Scale bar, 100 μm. Parts (b–d) are adapted with permission of American Society of Nephrology from [32]; permission conveyed through Copyright Clearance Center, Inc. This figure is available as part of a downloadable slideset