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. Author manuscript; available in PMC: 2019 Oct 1.
Published in final edited form as: Mech Ageing Dev. 2018 Jul 25;175:74–82. doi: 10.1016/j.mad.2018.07.008

Figure 1. Expression of Senescence Markers in HAART Drug Treated HUVECs.

Figure 1.

HUVECs were treated with 10 μg/mL of tenofovir (TDF) and emtricitibine (FTC) for 7 days. (A) Growth curve. (B) Population doublings over one week. (C) Left, representative SA β-gal images displayed at 20X of human astrocytes stained for SA β-gal 1 week after HAART treatment. Right, quantification of images. (D) Lysosomal mass was measured by incubation with Lysotracker for 30 min followed by quantification on a flow cytometer. (E) Left, representative western blot of senescence markers lamin B1 and loading control. Right, quantification. * = p value < .05, n = 3, error bars are S.D.