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. Author manuscript; available in PMC: 2019 Oct 1.
Published in final edited form as: Mech Ageing Dev. 2018 Jul 25;175:74–82. doi: 10.1016/j.mad.2018.07.008

Figure 5. Effect of p38MAPK Inhibition on HAART Drug Treated HUVECs.

Figure 5.

HUVECs were treated with 10 μg/mL of tenofovir (TDF) and emtricitibine (FTC) for 7 days. Where indicated, cells were pre-treated with 10 μM SB 203580 for 2 hours prior to HAART drug addition. (A) Lysosomal mass was measured by incubation with Lysotracker for 30 min followed by quantification on a flow cytometer. (B) Total ROS was measured by incubation with DCFDA for 30 min followed by quantification on a flow cytometer. (C) Mitochondrial ROS was measured by incubation with MitoSox for 30 min followed by quantification on a flow cytometer. * = p value < .05, n = 3, error bars are S.D.