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. Author manuscript; available in PMC: 2019 Oct 1.
Published in final edited form as: Mech Ageing Dev. 2018 Jul 25;175:74–82. doi: 10.1016/j.mad.2018.07.008

Figure 6. Nitric Oxide Activity in HAART Drug Treated HUVECs.

Figure 6.

HUVECs were treated with 10 μg/mL of tenofovir (TDF) and emtricitibine (FTC) for 7 days. Where indicated, cells were treated with 100 μM ATP one minute prior to collection. (A) Protein levels of p-eNOS and total eNOS as measured by western blot. (B) Quantification of A. (C) Intracellular nitric oxide was measured by incubation with DAF-FM and ATP for 30 min followed by a PBS wash and 15 min incubation before quantification on a flow cytometer. *= p value < .05 compared to (−) ATP control, # = p value < .05 compared to (−) ATP TDF FTC, n = 3, error bars are S.D.