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. Author manuscript; available in PMC: 2019 Oct 1.
Published in final edited form as: Mech Ageing Dev. 2018 Jul 25;175:74–82. doi: 10.1016/j.mad.2018.07.008

Figure 7. Effect of HUVEC Conditioned Media on Human Astrocytes.

Figure 7.

HUVECs were treated with 10 μg/mL of tenofovir (TDF) and emtricitibine (FTC) for 7 days. Cells were then incubated in human astrocyte media (HAM) for 24 hours to generate conditioned media (CM). CM was then incubated on human astrocytes for 7 days before indicated assays. (A) Representative SA Beta Gal assay images at 10X. (B) Quantification of A. (C) Total ROS was measured by incubation with DCFDA for 30 min followed by quantification on a flow cytometer. (D) Mitochondrial ROS was measured by incubation with MitoSox for 30 min followed by quantification on a flow cytometer. * = p value < .05, n = 3, error bars are S.D.