Figure 7.

NF-kB signaling modulated ROS in LPS induced alveolar epithelial mal-differentiation. (A, B): Flow cytometry data and merged image show LPS-induced ROS elevation in MLE-12 cells was abolished with Bay-11–7082. (D, C): In immunofluorescent staining of MLE-12 cells, red colour stands for Nrf2 and blue colour stands for DAPI staining, respectively. The higher magnification images are shown at the right upper corners. LPS-induced increase in Nrf2 expression was partially blocked with Bay-11–7082. (E): qPCR data show LPS induced alteration of oxidative stress related genes in MLE-12 cells, which were revered with Bay-11–7083. *P < 0.05. **P < 0.01. Scale bars = 50 μm.