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. 2018 Jul 31;17(14):1797–1811. doi: 10.1080/15384101.2018.1496748

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Figure 6. — SkQ1 influenced the distribution and activation of Aurora family kinases and Rb in НТ1080 cells. A. Aurora B distribution in the mitotic HT1080 cells after SkQ1 (40 nM, 24 h) treatment; blue – DNA, red – Aurora B; immunofluorescence microscopy. B. Measurements of Aurora B fluorescence intensity in the mitotic НТ1080 cells (М – Metaphase, Т – Telophase/Cytokinesis), SkQ1 (40 nM, 24 h), average fluorescence intensity/100 µm² ± SEM. C. SkQ1 (40 nM, 24 h) didn’t induce changes in mitotic cells number in HT1080 cells after colcemide (0.02 µg/ml, 24 h) treatment. D. Phospho-Auroras A, B, C and phospho-Rb (Ser807/811) in НТ1080 after colcemide (0.02 µg/ml, 24 h) and SkQ1 (100 nM, 24 h) treatment.