Figure 4.

Paip2 is associated with RNA in the nucleus.(a) Immunostaining of polytene chromosomes with antibodies against Paip2 and DAPI counterstaining without and after RNase treatment. (b) ChIP analysis of Paip2 levels in the DHR3 promoter region before and after induction by ecdysone and without and with RNase treatment. Designations are as in Figure 3C. (c, d) RNA immunoprecipitation (RIP) from lysate of S2 cells with antibodies against Paip2 and PABP and with empty beads (mock). The cells were either treated with ecdysone (DHR3 and DHR4 transcripts) or heat shock (hsp70 transcript). Primers for detecting transcripts were located in one exon within the coding region of the corresponding gene (C) or on both sides of the intron/exon boundary of the gene in order to detect nascent transcripts (D). RIP signals are given as percentage of input, enrichment of RNA relative to corresponding mock is indicated above the bars.