Table 1. Assessment of the interactions of Gal-Nab1 and Gal-Nab2 mAbs with gal-9M by surface plasmon resonance.
| Immobilized ligand | Gal-9M | Gal-Nab1 | Gal-Nab2 | |
|---|---|---|---|---|
| Analyte | Gal-Nab1 | Gal-Nab2 | Gal-9M | |
| KD (M) | (4.34 ± 1.24) x10-9 | (1.48x10-9 ± 5.30x10-10) | (8.87 ± 1.64) x10-8 | (8.79 ± 5.26) x10-9 |
| Kon (s-1 M-1) | (2.65x104 ± 1.86x103) | (6.11 ± 1.30) x104 | (3.74 ± 0.49) x105 | (1.90 ± 0.65) x105 |
| Koff (s-1) | (1.15x10-4 ± 2.87x105) | (9.06 ± 2.61) x10-5 | (3.32 ± 0.44) x10-2 | (1.67 ± 0.84) x10-3 |
| t1/2 (min) | 124.2 | 127.2 | 0.35 s | 7.32 |
KD, Kon and Koff were calculated as described under “Material and Methods”.