Skip to main content
. 2018 Sep 11;7:e38097. doi: 10.7554/eLife.38097

Figure 4. Functional characterization of ICAP2.

Figure 4.

(A) Schematic of the ICAP2 locus in the ICAP2-Ty cKD strain indicating the U1-mediated mRNA degradation following the rearrangement caused by a brief pulse of rapamycin (rapa). (B) Immunoblot of the ICAP2-Ty cKD strain monitoring degradation of ICAP2-Ty at different time points following a 2 hr pulse with rapa. ALD serves as a loading control. (C) At different time points following treatment with rapa or vehicle (N.T.), intracellular ICAP2-Ty cKD parasites were fixed and stained for Ty (green), ALD (red), and DAPI (blue). Scale bar is 10 µm. (D) Plaque assay of the parental and ICAP2-Ty cKD strains after treatment with rapa or a vehicle control (DMSO). (E) The parental and ICAP2-Ty cKD strains were pulsed with rapa or a vehicle control 24 hr prior to passaging. Samples were fixed 24 or 48 hr post infection (hpi) and stained for Ty and ALD. The distribution of parasites per vacuole was determined. Bars represent mean ± SD for n = 2 independent biological replicates. At least 150 vacuoles were counted per condition in 2 or more technical replicates. See also Figure 4—source data 1.

Figure 4—source data 1. This file contains the source data used to make the graph presented in Figure 4.
GraphPad Prism was utilized to visually represent the quantitative data.
elife-38097-fig4-data1.xlsx (11.2KB, xlsx)
DOI: 10.7554/eLife.38097.011