Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background (Toxplasma gondii) |
Parental (in Figures 1, 2 and 3) | PMID: 22144892 | TATi∆KU80 | |
| Strain, strain background (T. gondii) |
ICAP2-Ty | This paper | TATi∆KU80 ICAP2-Ty | |
| Strain, strain background (T. gondii) |
ICAP18-Ty | This paper | TATi∆KU80 ICAP18-Ty | |
| Strain, strain background (T. gondii) |
Parental in Figures 4 and 5 and 6 | PMID: 26090798 | DiCre∆KU80 | |
| Strain, strain background (T. gondii) |
ICAP2-Ty cKD | This paper | DiCre∆KU80 ICAP2-Ty cKD | |
| Strain, strain background (T. gondii) |
Parental strain (expressing GFP fused to the mitochondrial targeting signal of SOD2 and expressing the A3:E11 membrane complex protein IMC1 fused to TdTomato) |
This paper | DiCre∆KU80 SOD2-GFP IMC1-TdT | |
| Strain, strain background (T. gondii) |
ICAP2-Ty (cKD strain expressing GFP fused to the mitochondrial targeting signal of SOD2 and expressing the A3:E11 membrane complex protein IMC1 fused to TdTomato) |
This paper | DiCre∆KU80 ICAP2-Ty cKD SOD2-GFP IMC1-TdT | |
| Cell line (Homo sapiens) |
Human Foreskin Fibroblasts (HFFs) | ATCC | SCRC-1041 | |
| Antibody | Mouse monoclonal anti-Ty1 (clone BB2) |
PMID: 8813669 | Dilutions: IFA: 1/1000, WB: 1/10000 | |
| Antibody | Mouse monoclonal anti-TUB1 (clone 12G10) |
Developmental Studies Hybridoma Bank at the University of Iowa |
RRID: AB_1157911 | Dilution: WB 1/5000 |
| Antibody | Rabbit polyclonal anti-HSP70 | PMID: 17784785 | Dilutions: IFA: 1/1000, WB: 1/10000 | |
| Antibody | Rabbit polyclonal anti-ALD | PMID: 19380114 | Dilutions: IFA: 1/1000, WB: 1/10000 | |
| Antibody | Rabbit polyclonal anti-F1β | Agrisera | Agrisera:AS05085 | Dilution: WB 1/5000 |
| Antibody | Goat anti-Mouse IgG (H + L) Secondary Antibody, DyLight 488 conjugate |
Thermo Fisher | Thermo-Fisher:35502 | Dilutions: IFA: 1/1000 |
| Antibody | Goat anti-Rabbit IgG (H + L) Secondary Antibody, DyLight 594 conjugate |
Thermo Fisher | Thermo-Fisher:35560 | Dilutions: IFA: 1/1000 |
| Antibody | Peroxidase AffiniPure Goat Anti-Rabbit IgG (H + L) |
Jackson ImmunoResearch | Jackson ImmunoResearch:111-035-144 | Dilution: WB 1/10000 |
| Chemical compound, drug |
Hoechst | Santa Cruz | Santa Cruz:sc-394039 | Dilutions: IFA: 1/20000 |
| Chemical compound, drug |
Prolong Diamond | Thermo Fisher | Thermo-Fisher:P36965 | |
| Chemical compound, drug |
Gentamicin | Thermo Fisher | Thermo-Fisher:15710072 | |
| Chemical compound, drug |
Xanthine | Sigma-Aldrich | Sigma-Aldrich:X4002 | |
| Chemical compound, drug |
Mycophenolic Acid | Sigma-Aldrich | Sigma-Aldrich:M3536 | |
| Chemical compound, drug |
Rapamycin | EMD Millipore | EMD Millipore:553210 | |
| Chemical compound, drug |
Carbonyl cyanide 4- (trifluoromethoxy) phenylhydrazone (FCCP) |
Sigma-Aldrich | Sigma-Aldrich:C2920 | |
| Chemical compound, drug |
Antimycin A | Sigma-Aldrich | Sigma-Aldrich:A8674 | |
| Chemical compound, drug |
Atovaquone | Sigma-Aldrich | Sigma-Aldrich:A7986 | |
| Chemical compound, drug |
Oligomycin | EMD Milipore | EMD Millipore:495455 | |
| Chemical compound, drug |
2-Deoxy-D-glucose (2-DG) | Sigma-Aldrich | Sigma-Aldrich:D6134 | |
| Chemical compound, drug |
D-glucose | Thermo Fisher | Thermo-Fisher:15023021 | |
| Chemical compound, drug |
Glutamine | Sigma-Aldrich | Sigma-Aldrich:G8540 | |
| Sequence- based reagent |
All primers and oligonucleotides used in this study are listed in Supplementary file 2 |
This paper | ||
| Recombinant DNA reagent |
pU6-Universal | Addgene | Addgene:52694 | |
| Recombinant DNA reagent |
pT8mycSOD2(SPTP) GFPmycHX |
PMID: 17784785 | ||
| Recombinant DNA reagent |
TubIMC1TdTomato-CAT | PMID: 26845335 | ||
| Recombinant DNA reagent |
pG152-Lic-HA-FLAG-LoxP-3’UTRSag1-HXGPRT-LoxP-U1 | PMID: 26090798 | ||
| Recombinant DNA reagent |
pG152-ICAP2-HA | This paper | ||
| Peptide, recombinant protein |
Ty peptide | This paper | ||
| Commercial assay or kit |
Gibson Assembly Cloning Kit | New England Biolabs | NEB:E5510S | |
| Commercial assay or kit |
NucleoBond Xtra Midi | Macherey Nagel | Macherey Nagel:740412.50 | |
| Commercial assay or kit |
CellTiter-Glo Luminescent Cell Viability Assay |
Promega | Promega:15023021 | |
| Other | Pierce Protein G Magnetic Beads |
Thermo Fisher | Thermo-Fisher:88847 | |
| Other | glucose and glutamine-free DMEM |
Sigma-Aldrich | Silga-Aldrich:D5030 | |
| Other | FluoroBrite DMEM | Thermo Fisher | Thermo-Fisher:A1896701 | |
| Other | Halt protease inhibitor |
Thermo Fisher | Thermo-Fisher:862209 | |
| Other | CellTak cell adhesive |
In Vitro Technologies | In Vitro Technologies :FAL354240 |
|
| Software, algorithm |
HHPRED | PMID: 29258817 | ||
| Software, algorithm |
ClustalX | PMID: 17846036 | ||
| Software, algorithm |
Mascot, version 2.6.1 | Matrix Science | ||
| Software, algorithm |
Scaffold, version 4.8.3 | Proteome Software | ||
| Software, algorithm |
Prism, version 7 | Graphpad | ||
| Software, algorithm |
R, version 3.2.3 | R Foundation for Statistical Computing |
||
| Software, algorithm |
MitoGraph | PMID: 25640425 | ||
| Gene (T. gondii) | ICAP2 | N/A | ToxoDB:TGME49_231410 | |
| Gene (T. gondii) |
ICAP18 | N/A | ToxoDB:TGME49_268830 |