Figure 6.

Sucrose-loaded fibroblasts are characterized by an increased content of GD3 at both intracellular and PM levels. A) Indirect immunofluorescence of GD3 ganglioside obtained by staining with R24 antibody in human fibroblasts, loaded or not with sucrose permeabilized with Triton X-100. B) PM levels of GD3 ganglioside evaluated by indirect immunofluorescence in fibroblasts, loaded or not with sucrose with sucrose, in nonpermeabilizing conditions. Images are representative of 10 fields for each sample. Results represent 3 independent experiments performed in triplicate.