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. 2018 May 16;32(10):5724–5736. doi: 10.1096/fj.201800141R

Figure 6.

Figure 6

SFAs alter sphingolipid metabolism in intestinal epithelial cells. IEC6 cells were treated with 0.6 mM myristate (Myr), palmitate (PAL), vehicle (Veh) for 16 h. A–D) Individual species of sphingomyelin (A), S1P (B), C1P (C), and HexCer (D) were analyzed by electrospray ionization tandem mass spectrometry in the Stony Brook University Lipidomics Shared Resource Core and normalized to total lipid phosphate (Pi). E–I) IEC6 cells were pretreated 25 µM PDMP for 1 h, then treated with 0.6 mM Myr or Veh for 16 h. Total HexCer (E), C14-HexCer (F), and d18:1C14-ceramide (G) levels were analyzed and normalized to total lipid Pi. mRNA of ERdj4 (H) or IL-6 (I) were analyzed by quantitative RT-PCR and normalized to β-actin. Data represent means ± sem, n = 3. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 compared with Veh; ##P < 0.01, ####P < 0.0001 compared with DMSO/DMSO Myr treatment.