Figure 3.

Intracellular Cbl pool under experimental conditions of transcellular transport. Cells were incubated with [⁵⁷Co]-CNCbl-TC for 96 h, washed with PBS, and the Cbls were extracted and identified as previously published in Hannibal et al. (51). Each bar represents 12 pooled samples of the same experimental condition (samples were pooled to achieve a reproducible and reliable readout of radioactivity). Blue bars: The radioactive source was added to the upper compartment. Red bars: The radioactive source was added to the bottom compartment.