Figure 3.
WNT1 and MLPH are microRNA-5110 targets. A) The microRNA-5110 binding sites in the 3ʹ-UTRs of WNT1 and MLPH, as predicted by miRBase software. B) The sequences of the wild-type 3ʹ-UTRs of WNT1 and MLPH and of microRNA-5110. C) The relative expression of WNT1 and MLPH in alpaca skin with white and brown coat color. D, E) Analysis of WNT1 (D) and MLPH (E) protein expression in alpaca skin with white and brown coat color. F) Dual luciferase gene assay data, expressed as the mean relative luciferase activities ± sd (n = 3). *P < 0.05, **P < 0.01, ***P < 0.001.
