TABLE 1.
MicroRNAs and mRNAs primer sequences
| Primer sequence, 5′–3′ |
|||
|---|---|---|---|
| Gene | Forward | Reverse | Application |
| MLPH-WT | CGAGCTCTGTCACCTGTGCCCTGAACTGCTTG | GCCTCGAGGGTGGGTATGGTAAGGAACTCT | Luciferase reporter-WT |
| MLPH-mut | AAGCCCCATTGGTCCCAGGAAAGCAGGACC | TTCGGGGTAACCAGGGTCCTTTCGTCCTGG | Luciferase reporter-mut |
| WNT1-WT | GCGAGCTCTGTTGCGGTTCCTGATGT | GCCTCGAGCCTATGAGAAGCTGGGTAAA | Luciferase reporter-WT |
| WNT1-mut | ATGCCTCCCTCAGCCTGGACCCACCCCTTCCTG | TACGGAGGGAGTCGGACCTGGGTGGGGAAGGAC | Luciferase reporter-mut |
| MicroRNA-5110 | ACACTCCAGCTGGGGGAGGAGGTAGAGGGTGGT | TGGTGTCGTGGAGTCG | Real-time PCR |
| Common-R | CGAGCAGTGCAGGGTCCGAGGT | RT-PCR | |
| U6 | CTCGCTTCGGCAGCACA | GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACTCATCT | Real-time PCR |
| MLPH | ATACTTCTTCCCCGGCTTGT | CTGTTGGGGTTCCTCTGTGT | Real-time PCR |
| WNT1 | ACCTGCTGACCGATTCCAAG | ACAGCCTCGGTTGACGATTT | Real-time PCR |
| Rab27a | TCACGACAGTCGGCATTG | GCTCTGGCTTCTTCCTCT | Real-time PCR |
| Myo5a | GGACTAAGAAAGCGAACC | TGAGCAGCCTGAATGAGA | Real time PCR |
| MITF | TCCCAAGTCAAATGATCCAG | GAGCCTGCATTTCAAGTTCC | Real-time PCR |
| TYR | GCTTTAGCAACTTCATGGGA | CTTGTTCTTCTCTGGGACAC | Real time PCR |
| TYRP1 | GCCTTCTTTCTCCCTTC | CAGACCACTCGCCATT | Real-time PCR |
| TYRP2 | AGCAGACGGAACACTGGACT | GCATCTGTGGAAGGGTTGTT | Real-time PCR |
| β-Actin | CTAAGGAGAAGGGCCAGTCC | CTCAAGTTGGGGGACAAAAA | Real-time PCR |
WT, wild-type; mut, mutation.