Table 2.

Percentages of IFNγ producing and/or degranulating CD8⁺ T cells expressing chain pairing enhancing modified TCRs after stimulation with HCV APLs^a

		TCR
Peptide	Phenotype	Wildtype	Disulfide Bridge	Leucine Zipper	mCβ2
Tyrosinase	non-reactive	96.0	96.6	92.6	91.8
	IFNγ	1.1	1.0	2.6	2.9
	CD107a	2.3	1.9	3.3	3.4
	IFNγ & CD107a	0.6	0.4	1.9	1.9
HCV WT	non-reactive	28.7	35.4*	9.5**	7.9**
	IFNγ	15.5	11.0	13.6	14.3
	CD107a	13.4	27.0	12.5	10.1
	IFNγ & CD107a	42.5	26.7†	64.3†	67.7††
I1412Lb	non-reactive	30.7	36.7	10.7**	8.4**
	IFNγ	15.2	11.7	12.9	14.8
	CD107a	15.6	24.5	13.1	9.1
	IFNγ & CD107a	38.9	27.1†	63.4†	67.7††
V1408Tb	non-reactive	34.5	41.7*	12.9**	9.6**
	IFNγ	14.1	11.0	14.1	14.7
	CD107a	14.5	22.9	12.8	10.3
	IFNγ & CD107a	36.8	24.5†	60.2†	65.5††
V1408Lb	non-reactive	30.0	36.0*	10.4**	8.0**
	IFNγ	15.0	11.9	13.3	14.5
	CD107a	15.1	24.6	12.5	9.3
	IFNγ & CD107a	39.9	27.5†	63.9†	68.2††
I1412Vb	non-reactive	33.7	41.9*	10.6**	9.1**
	IFNγ	14.6	12.3	13.7	14.7
	CD107a	13.7	21.1	12.7	10.0
	IFNγ & CD107a	38.0	24.8†	62.9†	66.2††
A1409Tc	non-reactive	51.5	63.7*	23.0**	17.0**
	IFNγ	12.2	7.8	16.9	18.5
	CD107a	12.7	15.8	12.8	10.0
	IFNγ & CD107a	23.7	12.6††	47.3†	54.6††
I1412Nc	non-reactive	45.4	55.0*	16.0**	12.6**
	IFNγ	13.2	9.8	16.0	16.4
	CD107a	12.6	17.9	12.6	10.3
	IFNγ & CD107a	28.8	17.4†	55.3††	60.7††
8S/9G/12Lc	non-reactive	85.2	88.6	71.4*	64.0*
	IFNγ	4.3	3.4	9.2	11.1
	CD107a	5.6	5.4	7.5	8.3
	IFNγ & CD107a	4.9	2.6	11.9†	16.6†
8S/9S/12L/14Sc	non-reactive	51.3	62.7*	24.4**	19.3**
	IFNγ	11.6	8.1	16.0	16.6
	CD107a	13.1	15.9	13.4	11.7
	IFNγ & CD107a	24.0	13.2†	46.3†	52.4††

^aAverages of 3 independent experiments using 3 PBL donors (n=9)

^bModerate affinity APL;

^cLow affinity APL;

Significantly greater total percent reactive cells compared to WT TCR (significantly less non-reactive percentage) is denoted

^*p<0.05,

^**p<0.001;

Significantly greater frequency of bi-functional cells compared to WT TCR is denoted

^†p<0.05,

^††p<0.001.